Z-100, an immunomodulatory extract of Mycobacterium tuberculosis strain Aoyama B, prevents spontaneous lymphatic metastasis of B16-BL6 melanoma.

A clinically relevant, syngeneic model of spontaneous, highly metastatic B16 mouse melanoma.

We report a syngeneic model of spontaneous metastatic B16-F10 mouse melanoma in C57/BL6 mice with a very high metastatic frequency that mimics clinical metastatic melanoma. The B16 melanoma cells were injected between the skin and cartilage on the dorsal side of the ear. The model generated lymphatic and visceral metastases in all of the tested animals. In mice with large primary tumors, tumor ...

Inhibitory effect of cytostatin on spontaneous lung metastases of B16-BL6 melanoma cells.

Tumorcells may interact with extracellular matrices (ECM) such as laminin, fibronectin and collagen type IV, at a variety of stages in the metastatic cascade1'2). Some low molecular weight inhibitors of cell adhesion which reduce experimental and/or spontaneous lung metastases have been reported3 ~ 5). Recently, we have isolated a novel inhibitor of cell adhesion to components of ECM,namedcytos...

Use of Mycobacterium vaccae (SRLI72) as an Immunomodulating Agent in Association with Murine Melanoma Model B16-F10

B16-BL6 melanoma cells release inhibitory factor(s) of active pump activity in isolated lymph vessels.

We investigated whether supernatant cultured with melanoma cell lines B16-BL6 and K1735 or the Lewis lung carcinoma cell line (LLC) can regulate lymphatic pump activity with bioassay preparations isolated from murine iliac lymph vessels. B16-BL6 and LLC supernatants caused significant dilation of lymph microvessels with cessation of pump activity. B16-BL6 supernatant produced dose-related cessa...

An attempt to isolate genes responsible for spontaneous and experimental metastasis in the mouse model.

Cancer develops and progresses as genetic alterations occur subsequently. Onset process of cancer has become well understood in some types of cancer, such as colorectal cancers. In this process, responsible alterations were identified in numbers of oncogenes such as k-ras, and tumor suppressor genes such as p53, as Vogelstein proposed earlier in the multistage carcinogenesis theory. In contrast...